| Facility Equipment |
Femtosecond Transient Spectroscopy
Single Molecule Microscopy
Time-correlated Single Photon Counting Apparatus
Computers and Other Accessories
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Femtosecond Transient Spectroscopy
An overview of one of the laser labs (B348). There are four time-resolved spectroscopic setups in this lab – a fs broadband transient spectrometer, a fs single wavelength transient spectrometer, a fluorescence up-conversion setup, and a single molecule spectroscopic setup. |
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The broadband transient spectrometer consists of a KHz regeneratively amplified Ti:S laser and an OPA (Clark-MXR), (uper left). The optical setup is a pump-probe apparatus with a dual diode array detector. A cryostat (APD) can be used to perform temperature-dependent measurements from 10 K to 330 K. |
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| The second transient spectrometer in the facility utilizes a regeneratively amplified KHz Ti:S laser system from Spectra Physics (left). Two OPAs are used to extend the measuring capabilities, such as two-pulse excitation experiments, and pump-probe spectroscopy into the 300 – 400 nm region using single wavelength pump-probe detection techniques. |
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| Fluorescence up-conversion setup uses a femtosecond Ti:S laser as the light source, and an optical delayline to control the time delay. The apparatus measures excited state lifetimes of molecules with a femtosecond time resolution. |
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| Single Molecule Microscopy |
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Single molecule spectroscopy setup in B348 The single molecule microscope consists of an excitation laser, a confocal microscope base, high numerical aperture objective lens, and a sensitive detection system, which operates in single photon counting mode
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Single molecule spectroscopy setup located in A2C uses a Ti:S laser (Spectra Physics) to perform one or two-photon laser excitation. The single molecule/fluorescence lifetime imaging microscope (right) is used to measure the FRET at a single molecule level. |
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Time-correlated Single Photon Counting Apparatus
Time-correlated single photon counting apparatus is located in PS-A2E. It measures excited state lifetimes with a very high signal-to-noise ratio. Light sources include Nd:YAG laser (Coherent) pumped dye lasers (Coherent and Spectra Physics) and a Ti:S laser with a frequency doubler and pulse Picker (Spectra Physics).
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| Computers used for data acquisition and analysis. Experiment control and data acquisition software was developed using LabWin/CVI, the data analysis software, ASUFIT, was written in MatLab.
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| Other lab accessories include a spectrophotometer (Shimadzu), an Oxford liquid helium cryostat for measuring 77K to room temperature, and an APD closely circulated cryostat which can be used in the temperature region from 10 K to 330 K.
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