Representative Publications
"Chromogenic Cross-link Formation in Green Fluorescent Protein ," Wachter, R. M., Acc. Chem. Res. 40, 120-127 (2007).
"X-ray Structure of Cerulean GFP: A Tryptophan-Based Chromophore Useful for Fluorescence Lifetime Imaging," Malo, G. D., Pouwels, L. J., Wang, M., Weichsel, A., Montfort, W. R., Rizzo, M. A., Piston, D. W., Wachter, R. M., Biochemistry (Accelerated Publication) 46, 9865-9873 (2007).
"Reaction Progress of Chromophore Biogenesis in Green Fluorescent Protein," Zhang, L., Patel, H. N., Lappe, J. W., Wachter, R. M., J. Am. Chem. Soc. 128, 4766-4772 (2006).
"The family of GFP-like proteins: Structure, Function, Photophysics and Biosensor Applications," Wachter, R. M., Photochem. Photobiol. 82, 339-344 (2006).
"Maturation Efficiency, Trypsin Sensitivity and Optical Properties of Arg96, Glu222 and Gly67 Variants of Green Fluorescent Protein.," J.A. Sniegowski, M.E. Phail and R.M. Wachter, Biochem. Biophys. Res. Comm 332, 657-663 (2005).
"Oxidative Chemistry in the GFP Active Site Leads to Covalent Cross-Linking of a Modified Leucine Side Chain with a Histidine Imidazole: Implications for the Mechanism of Chromophore Formation," M.A. Rosenow, H.N. Patel and R.M. Wachter, Biochemistry 44, 8303-8311 (2005).
"Base-catalysis of Chromophore Fromation in Arg96 and Glu222 variants of Green Fluorescent Protein," J.A. Sniegowski, J.W. Lappe, H.N. Patel, H.A. Huffman and R.M. Wachter, J. Biol. Chem 280, 26248-26255 (2005).
"zFP538, a Yellow Fluorescent Protein from Zoanthus, Contains a Novel Three-Ring Chromophore," S.J. Remington, R.M. Wachter, D.K. Yarbrough, B.P. Branchaud, D.C. Anderson, K. Kallio and K.A. Lukyanov, Biochemistry 44, 202-212 (2005).
"X-ray diffraction analysis and molecular replacement solution of the cyan fluorescent protein dsFP483," M. Wang, H.N. Patel and R. Wachter, Acta Cryst. 61, 922-924 (2005).
"Development and Characterization of Green Fluorescent Protein Mutants with Altered Lifetimes," Scruggs, A. W., Flores, C. L., Wachter, R. M., Woodbury, N. W. , Biochemistry 44, 13377- 13384 (2005).
"The Crystal Structure of the Y66L Variant of Green Fluorescent Protein Supports a Cyclization-Oxidation-Dehydration Mechanism for Chromophore Maturation.," M.A. Rosenow, H.A. Huffman, M.E. Phail and R.M. Wachter, Biochemistry 43, 4464-4472 (2004).
"Light Driven Decarboxylation of Wild-Type Green Fluorescent Protein.," Bell, A. F., Stoner-Ma, D., Wachter, R. M., Tonge, P. J. , J. Am. Chem. Soc. 125, 6919-6926 (2003).
|