Department of Chemistry Biochemistry

HAO YAN

Milton D. Glick Distinguished Professor
Ph.D., New York University, 2001

2006 NSF Career Award
2006 AzTE Innovator of Tomorrow
2007 AFOSR Young Investigator Award
2008 Alfred P. Sloan Research Fellowship

  Office: BDA-110  Lab: Biodesign A 131
  Phone: (480)727-8570  Lab Phone: (480)727-0428
  Fax: (480) 965-2747
  Email: hao.yan@asu.edu

 Dr. HAO YAN's Lab or Group Website

Research and Teaching Interests

Our research program is highly interdisciplinary which combines Chemistry, Biology, Physics and Material Science. Our goal is to achieve programmed design and assembly of biologically inspired nanomaterials and to explore its applications in nanoelectronics, controlled macromolecular interactions and biosensing. Our research is focused in the following four themes:

1) Bio-Nanotechnology: Design of novel DNA nanostructures, implementation of the designed structure in the construction of patterned DNA arrays and nanomechanical devices. Develop modular methods to achieve biomimetic molecular motors.

2) Nanoelectronics: Utilize rationally designed DNA nanostructure to template nanoelectronic components such as nanoparticles or carbon nanotubes into functional nanodevices.

3) Macromolecule Structure Elucidation: Develop methods to self-assemble 2D and 3D protein arrays for structural determination using Electron Microscopy or X-ray Crystallography.

4) Biomolecular Imaging: Investigation of protein-DNA interactions using high resolution imaging technology such as Atomic Force Microscopy and Electron Microscopy.

Major techniques in our group include: DNA/RNA/Protein manipulation (gel electrophoresis, labeling, hybridization, PCR and footprinting, cloning), electron-beam lithography, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Electron Microscopy (EM), Fluorescence Spectroscopy, UV-Vis, Circular Dichroism (CD) and chemical synthesis.

 

In collaboration with Sean Williams (Programmer, now at UC Davis), Peter Wonka (ASU) and Stuart Lindsay (ASU), we developed a graphical user interface program for the design of DNA nanostructures and sequences. Please click to download this program and a user manual associated with it.  

Selected Publications

"Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures," J. Fu, M. Liu, Y. Liu, N. W. Woodbury, H. Yan, J. Am. Chem. Soc. 134 55165519 (2013)

"DNA Gridiron Nanostructures Based on Four-Arm Junctions," D. Han, S. Pal, Y. Yang, S. Jiang, J. Nangreave, Y. Liu, H. Yan, Science 339 1412-1415 (2013)

"DNA Origami with Complex Curvatures in Three-dimensional Space," D. Han, S. Pal, J. Nangreave, Z. Deng, Y. Liu, H. Yan, Science 332 342 - 346 (2011)

"Molecular Robots Guided by Prescriptive Landscapes," K. Lund, A. J. Manzo, N. Dabby, N. Michelotti, A. Johnson-Buck, J. Nangreave, S. Taylor, R. Pei, M. N. Stojanovic*, N. G. Walter*, E. Winfree*, H. Yan*, Nature 465 206-210 (2010)

"Folding and cutting DNA into reconfigur-able topological nanostructures," D. Han, S. Pal, Y. Liu*, H. Yan*, Nature Nanotechnology 5 712-717 (2010)

"Control of Self-Assembly of DNA Tubules Through Integration of Gold Nanoparticles," J. Sharma, R. Chhabra, A. Cheng, J. Brownell,Y. Liu, H. Yan, Science 323 112-116 (2009)

"Mirror Image DNA Nanostructures for Chiral Supramolecular Assemblies,," C. Lin, Y. Ke, Z. Li, J. Wang, Y. Liu*, H. Yan*, Nano Letters 9 433-436 (2009)

"Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays," Yonggang Ke, Stuart Lindsay,Yung Chang, Yan Liu, Hao Yan*, Science 319 180-183 (2008)

"In vivo cloning of artificial DNA nanostructures," C. Lin, S. Rinker, X. Wang, Y. Liu, N. C. Seeman, H. Yan, Proc. Natl. Acad. Sci 105 17626-17631 (2008)

"Quantum Dots Bioconjugation During Core-Shell Synthesis," Q. Wang, Y. Liu, Y. Ke, H. Yan, Angew Chem Int Ed. 47 316-319 (2008)

"DNA-Tile-Directed Self-assembly of Quantum Dots into Two-Dimensional Nanopatterns," J. Sharma, Y. Ke, C. Lin, R. Chhabra, Q. Wang, J. Nangreave, Y. Liu, H. Yan, Angew Chem Int Ed 47 5157-5159 (2008)

"Self-assembled DNA nanostructures for distance-dependent multivalent ligand-protein binding," S. Rinker, Y. Ke, Y. Liu*, R. Chhabra, H. Yan*, Nature Nanotechnology 3 418-422 (2008)

"A Facile One-step In situ Functionalization of Quantum Dots with Preserved Photoluminescence for Bioconjugation," Q. Wang, Y. Xu, X. Zhao, Y Chang, Y. Liu, L, Jiang, J. Sharma, D.-K, Seo*, H. Yan*, J. Am. Chem. Soc. 129 6380-6381 (2007)

"Spatially Addressable Multi-protein Nanoarrays Templated by Aptamer Tagged DNA Nanoarchitectures," Rahul Chhabra, Jaswinder Sharma, Yonggang Ke, Yan Liu, Sherri Rinker, Stuart Lindsay, Hao Yan*, J. Am. Chem. Soc. 129 10304-10305 (2007)

"Rolling Circle Enzymatic Replication of a Complex Multi-crossover DNA Nanostructure," C. Lin, X. Wang, Y. Liu, N. C. Seeman, H. Yan*, J. Am. Chem. Soc. 129 14475-14481 (2007)

"Self-assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing," C. Lin, Y. Liu, H. Yan*, Nano Letters 7 507-512 (2007)

"Mechanism of a Self-templating Synthesis of Monodispersed Hollow Silica Nanospheres with Tunable Size and Shell Thickness," Q. Wang, Y. Liu, H. Yan*, Chemical Communication 2339-2341 (2007)

"Functional DNA Nanotube Arrays: Bottom-up Meets Top-down," C. Lin, Y. Liu, M. Mertig, J. Gu, H. Yan*, Angew Chem Int Ed. 46 6089-6092 (2007)

"A Study of DNA Tube Formation Mechanisms Using 4-, 8-, and 12-Helix DNA Nanostructures," Y. Ke, Y. Liu, J. Zhang, H. Yan*, J. Am. Chem. Soc. 128 4414-4421 (2006)

"Self-assembled Signaling Aptamer Nanoarrays for Protein Detection," C. Lin, E. Katilius, Y. Liu, H. Yan*, Angew. Chem. Int. Ed. 45 5296-5301 (2006)

"DNA templated self-assembly of two-dimensional and periodical gold nanoparticle arrays," J. Sharma, R. Chhabra, Y. Liu, Y. Ke and H. Yan*, Angew. Chem. Int. Ed. 45 730-735 (2006)

"Periodic Square-like Gold Nanoparticle Arrays Templated by a DNA Nanogrids on a Surface ," J. Zhang, Y, Liu, Y. Ke, H. Yan*, Nano Lett. 6 248-252 (2006)

"Rolling Circle Amplification of a DNA Nano-junction," C. Lin, M. Xie, JL. Chen, Y. Liu, H. Yan*, Angew. Chem. Int. Ed. 45 7537-7539 (2006)

"Self-assembly of symmetric finite size DNA nanoarrays," Liu, Y. Ke and H. Yan*, J. Am. Chem. Soc. 127 17140-17141 (2005)

"Self-assembling a Molecular Pegboard," K. Lund, Y. Liu, S. Lindsay and H. Yan*, J. Am. Chem. Soc. 127 17606-17607 (2005)

"Aptamer-directed self-assembly of protein arrays on a DNA nanostructure," Y. Liu, C. Lin, H. Li and H. Yan*, Angew. Chem. Int. Ed 44 4333-4338 (2005)

"Programmable DNA Self-assemblies for Nanoscale Organization of Ligands and Proteins," S. H. Park, P. Yin, Y. Liu, J. Reif, T. H. LaBean, H. Yan* , Nano Lett. 5 729 (2005)

"Nucleic Acid Nanotechnology," H. Yan*, Science 306 2048-2049 (2004)

"DNA templated self-assembly of protein and nanoparticle linear arrays," H. Li, S.H. Park, J.H. Reif, T.H. LaBean and H. Yan*, J. Am. Chem. Soc. 126 418 (2004)

"DNA templated Self-assembly of Protein Arrays and Highly Conductive Nanowires," H. Yan*, S.H. Park, G. Ginkelstein, J.H. Reif and T.H. LaBean*, Science 301 1882 (2003)

" A two-state DNA lattice switched by DNA nanoactuator," L. Feng, S.H. Park, J.H. Reif and H. Yan*, Angew. Chem. Int. Ed. 42 4342 (2003)