 Research Interests
Our research program is highly interdisciplinary which combines Chemistry, Biology, Physics and Material Science. Our goal is to achieve programmed design and assembly of biologically inspired nanomaterials and to explore its applications in nanoelectronics, controlled macromolecular interactions and biosensing. Our research is focused in the following four themes:
1) Bio-Nanotechnology: Design of novel DNA nanostructures, implementation of the designed structure in the construction of patterned DNA arrays and nanomechanical devices. Develop modular methods to achieve biomimetic molecular motors.
2) Nanoelectronics: Utilize rationally designed DNA nanostructure to template nanoelectronic components such as nanoparticles or carbon nanotubes into functional nanodevices.
3) Macromolecule Structure Elucidation: Develop methods to self-assemble 2D and 3D protein arrays for structural determination using Electron Microscopy or X-ray Crystallography.
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Dr. Yan and his research group |
4) Biomolecular Imaging: Investigation of protein-DNA interactions using high resolution imaging technology such as Atomic Force Microscopy and Electron Microscopy.
Major techniques in our group include: DNA/RNA/Protein manipulation (gel electrophoresis, labeling, hybridization, PCR and footprinting, cloning), electron-beam lithography, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Electron Microscopy (EM), Fluorescence Spectroscopy, UV-Vis, Circular Dichroism (CD) and chemical synthesis.
Representative Publications
"Quantum Dots Bioconjugation During Core-Shell Synthesis," Q. Wang, Y. Liu, Y. Ke, H. Yan, Angew Chem Int Ed. 47, 316-319 (2008).
"Self-Assembled Water-Soluble Nucleic Acid Probe Tiles for Label-Free RNA Hybridization Assays," Yonggang Ke, Stuart Lindsay,Yung Chang, Yan Liu, Hao Yan*, Science 319, 180-183 (2008).
"Self-assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing," C. Lin, Y. Liu, H. Yan*, Nano Letters 7, 507-512 (2007).
"Self-assembled Peptide Nanoarrays: An approach to studying protein-protein interactions," B. Williams, K. Lund, Y. Liu, H. Yan*, J. Chaput*, Angew Chem Int Ed. 46, 3051-3054 (2007).
"Mechanism of a Self-templating Synthesis of Monodispersed Hollow Silica Nanospheres with Tunable Size and Shell Thickness," Q. Wang, Y. Liu, H. Yan*, Chemical Communication , 2339-2341 (2007).
"Functional DNA Nanotube Arrays: Bottom-up Meets Top-down," C. Lin, Y. Liu, M. Mertig, J. Gu, H. Yan*, Angew Chem Int Ed. 46, 6089-6092 (2007).
"A Facile One-step In situ Functionalization of Quantum Dots with Preserved Photoluminescence for Bioconjugation," Q. Wang, Y. Xu, X. Zhao, Y Chang, Y. Liu, L, Jiang, J. Sharma, D.-K, Seo*, H. Yan*, J. Am. Chem. Soc. 129, 6380-6381 (2007).
"Spatially Addressable Multi-protein Nanoarrays Templated by Aptamer Tagged DNA Nanoarchitectures," Rahul Chhabra, Jaswinder Sharma, Yonggang Ke, Yan Liu, Sherri Rinker, Stuart Lindsay, Hao Yan*, J. Am. Chem. Soc. 129, 10304-10305 (2007).
"Rolling Circle Enzymatic Replication of a Complex Multi-crossover DNA Nanostructure," C. Lin, X. Wang, Y. Liu, N. C. Seeman, H. Yan*, J. Am. Chem. Soc. 129, 14475-14481 (2007).
"DNA templated self-assembly of two-dimensional and periodical gold nanoparticle arrays," J. Sharma, R. Chhabra, Y. Liu, Y. Ke and H. Yan*, Angew. Chem. Int. Ed. 45, 730-735 (2006).
"A Study of DNA Tube Formation Mechanisms Using 4-, 8-, and 12-Helix DNA Nanostructures," Y. Ke, Y. Liu, J. Zhang, H. Yan*, J. Am. Chem. Soc. 128, 4414-4421 (2006).
"Self-assembled Signaling Aptamer Nanoarrays for Protein Detection," C. Lin, E. Katilius, Y. Liu, H. Yan*, Angew. Chem. Int. Ed. 45, 5296-5301 (2006).
"Rolling Circle Amplification of a DNA Nano-junction," C. Lin, M. Xie, JL. Chen, Y. Liu, H. Yan*, Angew. Chem. Int. Ed. 45, 7537-7539 (2006).
"Periodic Square-like Gold Nanoparticle Arrays Templated by a DNA Nanogrids on a Surface ," J. Zhang, Y, Liu, Y. Ke, H. Yan*, Nano Lett. 6, 248-252 (2006).
"Self-assembly of symmetric finite size DNA nanoarrays," Liu, Y. Ke and H. Yan*, J. Am. Chem. Soc. 127, 17140-17141 (2005).
"Self-assembling a Molecular Pegboard," K. Lund, Y. Liu, S. Lindsay and H. Yan*, J. Am. Chem. Soc. 127, 17606-17607 (2005).
"Aptamer-directed self-assembly of protein arrays on a DNA nanostructure," Y. Liu, C. Lin, H. Li and H. Yan*, Angew. Chem. Int. Ed 44, 4333-4338 (2005).
"Programmable DNA Self-assemblies for Nanoscale Organization of Ligands and Proteins," S. H. Park, P. Yin, Y. Liu, J. Reif, T. H. LaBean, H. Yan* , Nano Lett. 5, 729 (2005).
"Nucleic Acid Nanotechnology," H. Yan*, Science 306, 2048-2049 (2004).
"DNA templated self-assembly of protein and nanoparticle linear arrays," H. Li, S.H. Park, J.H. Reif, T.H. LaBean and H. Yan*, J. Am. Chem. Soc. 126, 418 (2004).
"DNA templated Self-assembly of Protein Arrays and Highly Conductive Nanowires," H. Yan*, S.H. Park, G. Ginkelstein, J.H. Reif and T.H. LaBean*, Science 301, 1882 (2003).
" A two-state DNA lattice switched by DNA nanoactuator," L. Feng, S.H. Park, J.H. Reif and H. Yan*, Angew. Chem. Int. Ed. 42, 4342 (2003).
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