Research and Teaching Interests
Our research program is highly interdisciplinary which combines Chemistry, Biology, Physics and Material Science. Our goal is to achieve programmed design and assembly of biologically inspired nanomaterials and to explore its applications in nanoelectronics, controlled macromolecular interactions and biosensing. Our research is focused in the following four themes:
1) Bio-Nanotechnology: Design of novel DNA nanostructures, implementation of the designed structure in the construction of patterned DNA arrays and nanomechanical devices. Develop modular methods to achieve biomimetic molecular motors.
2) Nanoelectronics: Utilize rationally designed DNA nanostructure to template nanoelectronic components such as nanoparticles or carbon nanotubes into functional nanodevices.
3) Macromolecule Structure Elucidation: Develop methods to self-assemble 2D and 3D protein arrays for structural determination using Electron Microscopy or X-ray Crystallography.
4) Biomolecular Imaging: Investigation of protein-DNA interactions using high resolution imaging technology such as Atomic Force Microscopy and Electron Microscopy.
Major techniques in our group include: DNA/RNA/Protein manipulation (gel electrophoresis, labeling, hybridization, PCR and footprinting, cloning), electron-beam lithography, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Electron Microscopy (EM), Fluorescence Spectroscopy, UV-Vis, Circular Dichroism (CD) and chemical synthesis.
|In collaboration with Sean Williams (Programmer, now at UC Davis), Peter Wonka (ASU) and Stuart Lindsay (ASU), we developed a graphical user interface program for the design of DNA nanostructures and sequences. Please click to download this program and a user manual associated with it.
"Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures," J. Fu, M. Liu, Y. Liu, N. W. Woodbury, H. Yan, J. Am. Chem. Soc. 134 5516–5519 (2013)
"DNA Gridiron Nanostructures Based on Four-Arm Junctions," D. Han, S. Pal, Y. Yang, S. Jiang, J. Nangreave, Y. Liu, H. Yan, Science 339 1412-1415 (2013)
"DNA Origami with Complex Curvatures in Three-dimensional Space," D. Han, S. Pal, J. Nangreave, Z. Deng, Y. Liu, H. Yan, Science 332 342 - 346 (2011)
"Molecular Robots Guided by Prescriptive Landscapes," K. Lund, A. J. Manzo, N. Dabby, N. Michelotti, A. Johnson-Buck, J. Nangreave, S. Taylor, R. Pei, M. N. Stojanovic*, N. G. Walter*, E. Winfree*, H. Yan*, Nature 465 206-210 (2010)
"Folding and cutting DNA into reconfigur-able topological nanostructures," D. Han, S. Pal, Y. Liu*, H. Yan*, Nature Nanotechnology 5 712-717 (2010)